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pDONR P4-P1R-EGFP
(Plasmid #48348)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48348 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDONRP4-P1R
  • Backbone manufacturer
    Life Technologies/Invitrogen
  • Backbone size w/o insert (bp) 2655
  • Total vector size (bp) 3363
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    Synthetic; Aequorea victoria
  • Insert Size (bp)
    708
  • Mutation
    Contains a Kozak sequence. Does not contain a stop codon. Aminoacid 40 is valine in our sequence, while it is phenylalanine in other EGFP sequences. This does not seem to affect fluorescence.
  • Promoter none

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 forward (-20)
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR P4-P1R-EGFP was a gift from Anna Planas & Tomas Santalucia (Addgene plasmid # 48348 ; http://n2t.net/addgene:48348 ; RRID:Addgene_48348)
  • For your References section:

    A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector. Buj R, Iglesias N, Planas AM, Santalucia T. BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18. 10.1186/1471-2199-14-18 PubMed 23957834