A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector.
Buj R, Iglesias N, Planas AM, Santalucia T
BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18. PubMed Article
Buj R, Iglesias N, Planas AM, Santalucia T
BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48344 | pDONR P4-P1R-mKate2 | Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins. |
| 48345 | pDONR P2R-P3-mKate2 | Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins. |
| 48346 | pDONR P4-P1R-V5-6xHis | Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins. |
| 48347 | pDONR P2R-P3-V5-6xHis | Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins. |
| 48348 | pDONR P4-P1R-EGFP | Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins. |
| 48349 | pDONR P2R-P3-EGFP | Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins. |
| 48350 | pDONR P4-P1R-EYFP | Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins. |
| 48351 | pDONR P2R-P3-EYFP | Contributes the coding sequence for EYFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins. |
| 48352 | pDONR P4-P1R-ECFP | Contributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins. |
| 48353 | pDONR P2R-P3-ECFP | Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins. |
| 48354 | pDONR P2R-P3-IRES_ECFP | Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules. |
| 48627 | pDONR P2R-P3-SV40 polyA signal | Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required. |