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pDONR P4-P1R-V5-6xHis
(Plasmid #48346)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48346 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDONRP4-P1R
  • Backbone manufacturer
    Life Technologies/Invitrogen
  • Backbone size w/o insert (bp) 2645
  • Total vector size (bp) 2745
  • Vector type
    Gateway entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V5 and 6xHis epitope tags cassette
  • Species
    Synthetic
  • Insert Size (bp)
    100
  • Mutation
    Contains a Kozak sequence and initiation codon upstream of V5 tag. Does not contain a stop codon.
  • Promoter none

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 forward (-20)
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Life Technologies
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR P4-P1R-V5-6xHis was a gift from Anna Planas & Tomas Santalucia (Addgene plasmid # 48346 ; http://n2t.net/addgene:48346 ; RRID:Addgene_48346)
  • For your References section:

    A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector. Buj R, Iglesias N, Planas AM, Santalucia T. BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18. 10.1186/1471-2199-14-18 PubMed 23957834