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(Plasmid #48354)


Item Catalog # Description Quantity Price (USD)
Plasmid 48354 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Life Technologies/Invitrogen
  • Backbone size w/o insert (bp) 2642
  • Total vector size (bp) 4058
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Synthetic; Aequorea victoria
  • Insert Size (bp)
  • Mutation
    ECFP translation is mediated by an IRES. Contains a stop codon at the end of the ECFP sequence. There is a nucleotide difference at position 1265 between our sequencing of this plasmid (A) and the reported sequence for the pYIC plasmid used as template for the PCR amplification of the insert (G). We have not confirmed whether this is related to the PCR. Nevertheless this is part of the IRES sequence and not the ECFP coding sequence.
  • Promoter none

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 forward (-20)
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR P2R-P3-IRES_ECFP was a gift from Anna Planas & Tomas Santalucia (Addgene plasmid # 48354 ; ; RRID:Addgene_48354)
  • For your References section:

    A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector. Buj R, Iglesias N, Planas AM, Santalucia T. BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18. 10.1186/1471-2199-14-18 PubMed 23957834