pAMHDAdGT8-4
(Plasmid #26421)

• Depositing Lab: Kohnosuke Mitani
• Publication: helper-dependent adenoviral vector (unpublished)

Backbone

• Vector backbone: pBR322
• Backbone size w/o insert (bp): 3360
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HSV-TK, mCMV-b-gal
• Species: E.coli, HSV
• Insert Size (bp): 8240

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI, SpeI, NheI, SalI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are some discrepancies between Addgene's quality control sequence and the author's sequence, but the Mitani lab has confirmed that this plasmid is correct. The depositor has also confirmed that there is an extra SacI site present in the plasmid is not apparent in the theoretical sequence on which the map below is based. Addgene does not recommend using SacI for a diagnostic digest.