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pEGFP-PCNA-IRES-puro2b
(Plasmid #26461)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26461 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIRES-puro2b
  • Backbone size w/o insert (bp) 6037
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCNA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    758
  • GenBank ID
    NP_002583.1
  • Entrez Gene
    PCNA (a.k.a. ATLD2)
  • Tag / Fusion Protein
    • pEGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that there are a few minor discrepancies between Addgene's quality control sequence and the author's sequence, but these should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-PCNA-IRES-puro2b was a gift from Daniel Gerlich (Addgene plasmid # 26461 ; http://n2t.net/addgene:26461 ; RRID:Addgene_26461)
  • For your References section:

    CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. Held M, Schmitz MH, Fischer B, Walter T, Neumann B, Olma MH, Peter M, Ellenberg J, Gerlich DW. Nat Methods. 2010 Sep . 7(9):747-54. 10.1038/nmeth.1486 PubMed 20693996