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MGP M155
(Plasmid #26527)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 26527 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pMSCV puro
  • Backbone size w/o insert (bp) 6300
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    BIC (miRNA containing region of Exon2)
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mir155 (a.k.a. Mirn155, mir-155, mmu-mir-155)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pLXSN-5
  • 3′ sequencing primer MSCV-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pMGP is a modified MSCV vector whereby GFP was placed downstream from the 5′ LTR, and the miR-155 expression cassette was cloned downstream from the GFP stop codon.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MGP M155 was a gift from David Baltimore (Addgene plasmid # 26527 ; ; RRID:Addgene_26527)
  • For your References section:

    Inositol phosphatase SHIP1 is a primary target of miR-155. O'Connell RM, Chaudhuri AA, Rao DS, Baltimore D. Proc Natl Acad Sci U S A. 2009 Apr 28. 106(17):7113-8. 10.1073/pnas.0902636106 PubMed 19359473