pBS-H19-luciferase ME
(Plasmid
#26613)
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Depositing Lab
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Depositing OrganizationUniversity of Illinois at Urbana-Champaign
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 26613-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 26613-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBS-H19-luciferase
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Backbone manufacturerShirley Tilghman lab
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIgf2 enhancer
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SpeciesM. musculus (mouse)
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Insert Size (bp)4000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (created at XhoI) (not destroyed)
- 3′ cloning site EagI (created at XhoI) (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNrev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 26613-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 26613-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-H19-luciferase ME was a gift from Jie Chen (Addgene plasmid # 26613 ; http://n2t.net/addgene:26613 ; RRID:Addgene_26613) -
For your References section:
IGF-II transcription in skeletal myogenesis is controlled by mTOR and nutrients. Erbay E, Park IH, Nuzzi PD, Schoenherr CJ, Chen J. J Cell Biol. 2003 Dec 8. 163(5):931-6. 10.1083/jcb.200307158 PubMed 14662739