|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26640||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5443
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)295
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
Expresses with RJ1878 ( Kan. resistant ) which is HU-free expression strain from BL21(DE3) family, from Reid Johnson.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET21a AHU was a gift from Phoebe Rice (Addgene plasmid # 26640 ; http://n2t.net/addgene:26640 ; RRID:Addgene_26640)
For your References section:Flexible DNA bending in HU-DNA cocrystal structures. Swinger KK, Lemberg KM, Zhang Y, Rice PA. EMBO J. 2003 Jul 15. 22(14):3749-60. 10.1093/emboj/cdg351 PubMed 12853489