Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET21a AHU
(Plasmid #26640)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26640 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AHU
  • Species
    Anabaena
  • Insert Size (bp)
    295

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Expresses with RJ1878 ( Kan. resistant ) which is HU-free expression strain from BL21(DE3) family, from Reid Johnson.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a AHU was a gift from Phoebe Rice (Addgene plasmid # 26640 ; http://n2t.net/addgene:26640 ; RRID:Addgene_26640)
  • For your References section:

    Flexible DNA bending in HU-DNA cocrystal structures. Swinger KK, Lemberg KM, Zhang Y, Rice PA. EMBO J. 2003 Jul 15. 22(14):3749-60. 10.1093/emboj/cdg351 PubMed 12853489