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pGEM-3z/605
(Plasmid #26659)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26659 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-3Z
  • Backbone manufacturer
    Promega
  • Backbone size (bp) 2743
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HincII (unknown if destroyed)
  • 3′ cloning site HincII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This plasmid contains a 147 bp nucleosome positioning sequence and is referred to as clone"605".

Please note that there may be discrepancies between Addgene's sequencing results and the depositor's sequence. Potential discrepancies are not a concern according to the depositing scientist because the clones were selected for their ability to bind to histone octamer, not for their specific sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-3z/605 was a gift from Jonathan Widom (Addgene plasmid # 26659 ; http://n2t.net/addgene:26659 ; RRID:Addgene_26659)
  • For your References section:

    New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning. Lowary PT, Widom J. J Mol Biol. 1998 Feb 13. 276(1):19-42. 10.1006/jmbi.1997.1494 PubMed 9514715