Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26792||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)900
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byMatthew H. Porteus
Terms and Licenses
Articles Citing this Plasmid
The original paper describing this plasmid should also be cited: Pruett-Miller, S.M., Connelly, J.P., Maeder, M.L., Joung, J.K., and Porteus, M.H. (2008). Comparison of zinc finger nucleases for use in gene targeting in mammalian cells. Mol. Ther. 16, 707–717.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GFPZFN2-B2H was a gift from Linzhao Cheng (Addgene plasmid # 26792 ; http://n2t.net/addgene:26792 ; RRID:Addgene_26792)
For your References section:Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells. Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, Cheng L. Cell Stem Cell. 2009 Jul 2. 5(1):97-110. 10.1016/j.stem.2009.05.023 PubMed 19540188