Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)
(Plasmid #26797)

• Purpose: Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Expresses tTA under EF1α promoter separately.
• Depositing Lab: Edward Hsiao
• Publication: Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Backbone

• Vector backbone: pcDNA3.2
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4879
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Rs1
• Species: H. sapiens (human)
• Insert Size (bp): 8047
• Mutation: D100A
• Entrez Gene: HTR4 (a.k.a. 5-HT4, 5-HT4R)
• Promoter: short TetO, miniCMV
• Tag / Fusion Protein:
  • mCherry-P2A (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Gene/Insert 2

• Gene/Insert name: tTA
• Promoter: EF1α

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pcDNA3.2 expression vector with the majority of the CMV promoter deleted, and carrying the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences