|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26881||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typePCR tagging yeast
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)230
- Cloning method Restriction Enzyme
- 5′ cloning site Pac1 (not destroyed)
- 3′ cloning site Asc1 (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
There is a short deletion in Addgene's QC sequence relative to the depositor's sequence, but it does not appear to affect the important features.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFA6a-BIO-TRP1 was a gift from Peter Kaiser (Addgene plasmid # 26881 ; http://n2t.net/addgene:26881 ; RRID:Addgene_26881)
For your References section:HB tag modules for PCR-based gene tagging and tandem affinity purification in Saccharomyces cerevisiae. Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, Auer B, Huang L, Kaiser P. Yeast. 2006 Jun . 23(8):623-32. 10.1002/yea.1380 PubMed 16823883