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(Plasmid #26901)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 26901 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5840
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Insert Size (bp)

Cloning Information

Resource Information

Depositor Comments

This is a single ORF (AgeI) Kozak-eGFP-CAAX-PTVI-2A-mCherry Sopt (ClaI).

AgeI and ClaI were added by PCR when generating the tg and those sites are not noted in the MCS of EF6V5.

Made as TOPO TA PCR cloning. Use SpeI/XbaI to validate insert in vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6V5:eGFP-CAAX-2A-mCherry was a gift from Steven Leach (Addgene plasmid # 26901 ; ; RRID:Addgene_26901)
  • For your References section:

    Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos. Provost E, Rhee J, Leach SD. Genesis. 2007 Oct . 45(10):625-9. 10.1002/dvg.20338 PubMed 17941043