|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26901||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5840
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1533
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
This is a single ORF (AgeI) Kozak-eGFP-CAAX-PTVI-2A-mCherry Sopt (ClaI).
AgeI and ClaI were added by PCR when generating the tg and those sites are not noted in the MCS of EF6V5.
Made as TOPO TA PCR cloning. Use SpeI/XbaI to validate insert in vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEF6V5:eGFP-CAAX-2A-mCherry was a gift from Steven Leach (Addgene plasmid # 26901 ; http://n2t.net/addgene:26901 ; RRID:Addgene_26901)
For your References section:Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos. Provost E, Rhee J, Leach SD. Genesis. 2007 Oct . 45(10):625-9. 10.1002/dvg.20338 PubMed 17941043
Map uploaded by the depositor.