|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26946||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeWorm Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namehistone H2B
SpeciesC. elegans (nematode)
Entrez Genehis-58 (a.k.a. CELE_F54E12.4)
/ Fusion Protein
- GFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
1st generation pie-1 vector
Note that there are some discrepancies between the Addgene QC sequence and the depositor's sequence. They should not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJH4.52 was a gift from Geraldine Seydoux (Addgene plasmid # 26946 ; http://n2t.net/addgene:26946 ; RRID:Addgene_26946)
For your References section:Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos. Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G. Dev Biol. 2008 Nov 1. 323(1):76-87. 10.1016/j.ydbio.2008.07.008 PubMed 18692039