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pRetroSuper-shYes
(Plasmid #26987)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26987 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRetroSuper
  • Backbone size w/o insert (bp) 6401
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA Yes
  • Alt name
    Yes
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    64
  • Entrez Gene
    YES1 (a.k.a. HsT441, P61-YES, Yes, c-yes)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pLXSN-5
  • 3′ sequencing primer MSCV-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

shRNA sequence - 5'-GTGACAATGTGAAACACTA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroSuper-shYes was a gift from Joan Massague (Addgene plasmid # 26987 ; http://n2t.net/addgene:26987 ; RRID:Addgene_26987)
  • For your References section:

    Latent bone metastasis in breast cancer tied to Src-dependent survival signals. Zhang XH, Wang Q, Gerald W, Hudis CA, Norton L, Smid M, Foekens JA, Massague J. Cancer Cell. 2009 Jul 7. 16(1):67-78. 10.1016/j.ccr.2009.05.017 PubMed 19573813