|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27083||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGE Healthcare Life Sciences
- Backbone size w/o insert (bp) 4952
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Alt nameCasein Kinase II alpha
SpeciesH. sapiens (human)
Insert Size (bp)1429
Entrez GeneCSNK2A1 (a.k.a. CK2A1, CKII, Cka1, Cka2, OCNDS)
/ Fusion Protein
- GST (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDB1 (CK2alpha) was a gift from David Litchfield (Addgene plasmid # 27083 ; http://n2t.net/addgene:27083 ; RRID:Addgene_27083)
For your References section:Protein kinase CK2 is a constitutively active enzyme that promotes cell survival: strategies to identify CK2 substrates and manipulate its activity in mammalian cells. Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, Litchfield DW.. Methods Enzymol. 2010;484:471-93. 10.1016/B978-0-12-381298-8.00023-X PubMed 21036246