Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27176||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationDeletion of 100 aa from ATG
Entrez GeneMRTFA (a.k.a. BSAC, MAL, MKL, MKL1, MRTF-A)
/ Fusion Protein
- 3XFLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Has no RPEL actin binding domain and is nuclear
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3XFLAG-MKL1-~100 was a gift from Ron Prywes (Addgene plasmid # 27176 ; http://n2t.net/addgene:27176 ; RRID:Addgene_27176)
For your References section:Megakaryoblastic leukemia 1, a potent transcriptional coactivator for serum response factor (SRF), is required for serum induction of SRF target genes. Cen B, Selvaraj A, Burgess RC, Hitzler JK, Ma Z, Morris SW, Prywes R. Mol Cell Biol. 2003 Sep . 23(18):6597-608. 10.1128/MCB.23.18.6597-6608.2003 PubMed 12944485
Map uploaded by the depositor.