pDestTol2pA2_ubi:EGFP
(Plasmid #27323)

• Purpose: MultiSite Gateway assembly vector, to generate transgenic zebrafish with ubiquitous expression of EGFP
• Depositing Lab: Leonard Zon
• Depositing Organization: Boston Children's Hospital
  Located in United States of America
• Publication: Mosimann et al Development. 2011 Jan . 138(1):169-77.
• Citations: 5 Articles

Backbone

• Vector backbone: pDestTol2A2
• Backbone size w/o insert (bp): 4179
• Vector type: Tol2 transposon

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ubiquitin:EGFP reporter
• Alt name: ubi:EGFP
• Species: D. rerio (zebrafish)
• Insert Size (bp): 4606
• Promoter: –3.5 kb zebrafish ubiquitin (ubi) promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: attB4 (not destroyed)
• 3′ cloning site: attB3 (not destroyed)
• 5′ sequencing primer: attB4
• 3′ sequencing primer: attB3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a few nucleotide discrepancies between author sequence and Addgene quality control sequence. According to the depositing lab, these do not affect plasmid function.

DNA (Catalog # 27323-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 27323-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.