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(Plasmid #27548)


Item Catalog # Description Quantity Price (USD)
Plasmid 27548 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Pseudomonas phage PP7 coat protein
  • Alt name
  • Species
    Pseudomonas phage PP7
  • Insert Size (bp)
  • Mutation
    changed two cysteine to alanine (C68A,C73A) to inhibit coat protein polymerization into capsid
  • GenBank ID
  • Entrez Gene
    cp (a.k.a. PP7p2)
  • Tags / Fusion Proteins
    • tandem protein A (ZZ) (N terminal on insert)
    • TEV protease cleavage site (N terminal on insert)
    • six histidine tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 Fwd
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETZZtevPP7CP(C68A,C73A) was a gift from Kathleen Collins (Addgene plasmid # 27548 ; ; RRID:Addgene_27548)
  • For your References section:

    RNA-based affinity purification reveals 7SK RNPs with distinct composition and regulation. Hogg JR, Collins K. RNA. 2007 Jun . 13(6):868-80. 10.1261/rna.565207 PubMed 17456562