pUHD Cdk1 DN HA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27653||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerM. Gossen et al., 1992 (PNAS)
- Backbone size w/o insert (bp) 3150
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationD146N (Dominant Negative)
Entrez GeneCDK1 (a.k.a. CDC2, CDC28A, P34CDC2)
/ Fusion Protein
- HA (YDVPDYA) (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer LNCX
- 3′ sequencing primer EBV_Rev (Common Sequencing Primers)
See attachment for description of backbone.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUHD Cdk1 DN HA was a gift from Greg Enders (Addgene plasmid # 27653 ; http://n2t.net/addgene:27653 ; RRID:Addgene_27653)