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(Plasmid #27707)


Item Catalog # Description Quantity Price (USD)
Plasmid 27707 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3961
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    caveolin-1, N-terminal truncated (60-178)
  • Alt name
    CAV1, cav-1
  • Species
    Canis lupus familiaris
  • Insert Size (bp)
  • Mutation
    N-terminal truncation with AA 1-59 missing. The resulting sequence is CAV1-(60-178).
  • GenBank ID
  • Entrez Gene
    CAV1 (a.k.a. caveolin 1)
  • Tag / Fusion Protein
    • monomeric EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer ACC TCT ACA AAT GTG GTA TGG CTG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This N-terminally truncated CAV1 forms 8S, but not 70S caveolar complexes. This results in delivery of unassembled CAV1 to the plasma membrane.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAV1-deltaN-mEGFP was a gift from Ari Helenius (Addgene plasmid # 27707 ; ; RRID:Addgene_27707)
  • For your References section:

    Biogenesis of caveolae: stepwise assembly of large caveolin and cavin complexes. Hayer A, Stoeber M, Bissig C, Helenius A. Traffic. 2010 Mar . 11(3):361-82. 10.1111/j.1600-0854.2009.01023.x PubMed 20070607