|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27707||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3961
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecaveolin-1, N-terminal truncated (60-178)
Alt nameCAV1, cav-1
SpeciesCanis lupus familiaris
Insert Size (bp)1109
MutationN-terminal truncation with AA 1-59 missing. The resulting sequence is CAV1-(60-178).
Entrez GeneCAV1 (a.k.a. caveolin 1)
/ Fusion Protein
- monomeric EGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer ACC TCT ACA AAT GTG GTA TGG CTG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This N-terminally truncated CAV1 forms 8S, but not 70S caveolar complexes. This results in delivery of unassembled CAV1 to the plasma membrane.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CAV1-deltaN-mEGFP was a gift from Ari Helenius (Addgene plasmid # 27707 ; http://n2t.net/addgene:27707 ; RRID:Addgene_27707)
For your References section:Biogenesis of caveolae: stepwise assembly of large caveolin and cavin complexes. Hayer A, Stoeber M, Bissig C, Helenius A. Traffic. 2010 Mar . 11(3):361-82. 10.1111/j.1600-0854.2009.01023.x PubMed 20070607