Skip to main content
Addgene
Open Menu

cavin-3-HA
(Plasmid #27711)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27711 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 27711-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 27711-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pHA-N-DEST
  • Backbone manufacturer
    Helenius lab
  • Backbone size w/o insert (bp) 5502
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cavin-3
  • Alt name
    SRBC
  • Alt name
    PRKCDBP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    783
  • Mutation
    R8P
  • GenBank ID
    NM_145040
  • Entrez Gene
    CAVIN3 (a.k.a. HSRBC, PRKCDBP, SRBC, cavin-3)
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attL1/attR1 (destroyed during cloning)
  • 3′ cloning site attL2/attR2 (destroyed during cloning)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mutation R8P is a known natural variant of cavin-3 (http://www.uniprot.org/uniprot/Q969G5) The cavin-3 cDNA used in this plasmid originated from the ORFeome library.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cavin-3-HA was a gift from Ari Helenius (Addgene plasmid # 27711 ; http://n2t.net/addgene:27711 ; RRID:Addgene_27711)

  • For your References section:

    Biogenesis of caveolae: stepwise assembly of large caveolin and cavin complexes. Hayer A, Stoeber M, Bissig C, Helenius A. Traffic. 2010 Mar . 11(3):361-82. 10.1111/j.1600-0854.2009.01023.x PubMed 20070607
Related items:
Commonly requested with: