cavin-3-HA
(Plasmid #27711)

• Depositing Lab: Ari Helenius
• Publication: Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Backbone

• Vector backbone: pHA-N-DEST
• Backbone manufacturer: Helenius lab
• Backbone size w/o insert (bp): 5502
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cavin-3
• Alt name: SRBC
• Alt name: PRKCDBP
• Species: H. sapiens (human)
• Insert Size (bp): 783
• Mutation: R8P
• GenBank ID: NM_145040
• Entrez Gene: CAVIN3 (a.k.a. HSRBC, PRKCDBP, SRBC, cavin-3)
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attL1/attR1 (destroyed during cloning)
• 3′ cloning site: attL2/attR2 (destroyed during cloning)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mutation R8P is a known natural variant of cavin-3 (http://www.uniprot.org/uniprot/Q969G5) The cavin-3 cDNA used in this plasmid originated from the ORFeome library.