Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28092||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt nameliver receptor homolog-1
SpeciesM. musculus (mouse)
Entrez GeneNr5a2 (a.k.a. AU020803, D1Ertd308e, Ftf, LRH-1, UF2-H3B)
/ Fusion Protein
- myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEF1a-myc-LRH1(ES)-IRES-neo was a gift from Austin Cooney (Addgene plasmid # 28092 ; http://n2t.net/addgene:28092 ; RRID:Addgene_28092)
For your References section:Canonical Wnt/I. Wagner RT, Xu X, Yi F, Merrill BJ, Cooney AJ. Stem Cells. 2010 Oct . 28(10):1794-804. 10.1002/stem.502 PubMed 20734354