pET-s34T
(Plasmid #28256)

• Depositing Lab: Yawen Bai
• Publication: Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Backbone

• Vector backbone: pET42+b
• Backbone size w/o insert (bp): 6000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Plasmid is provided in DH5a cells. BL21-CodonPlus (DE3)-RILP Competent Cells from Agilent are recommended for expression.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: single chain H3-H4 with Thrombin site
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 400
• Mutation: A thrombin cutting site between H3 and H4
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 pro
• 3′ sequencing primer: T7 ter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry