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pCMV-NHERF1-PDZ1iP
(Plasmid #28295)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28295 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NHERF1
  • Species
    H. sapiens (human)
  • Mutation
    Contains aa 1-148 (deletion of PDZ2 and EB domains)
  • Entrez Gene
    SLC9A3R1 (a.k.a. EBP50, NHERF, NHERF-1, NHERF1, NPHLOP2)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-NHERF1-PDZ1iP was a gift from Maria-Magdalena Georgescu (Addgene plasmid # 28295 ; http://n2t.net/addgene:28295 ; RRID:Addgene_28295)
  • For your References section:

    NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands. Morales FC, Takahashi Y, Momin S, Adams H, Chen X, Georgescu MM. Mol Cell Biol. 2007 Apr . 27(7):2527-37. 10.1128/MCB.01372-06 PubMed 17242191