Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28294||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationContains aa 1-298 (deletion of the EB domain aa 299-358)
Entrez GeneSLC9A3R1 (a.k.a. EBP50, NHERF, NHERF-1, NHERF1, NPHLOP2)
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
This plasmid contains a mutation (N268S) in the NHERF1 sequence, which corresponds to the mouse NHERF1 sequence at this position. The depositing scientist states that the mutation does not affect NHERF1 function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-NHERF1-PDZ1-2 was a gift from Maria-Magdalena Georgescu (Addgene plasmid # 28294 ; http://n2t.net/addgene:28294 ; RRID:Addgene_28294)
For your References section:NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands. Morales FC, Takahashi Y, Momin S, Adams H, Chen X, Georgescu MM. Mol Cell Biol. 2007 Apr . 27(7):2527-37. 10.1128/MCB.01372-06 PubMed 17242191