Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCMV-NHERF1-FL
(Plasmid #28291)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28291 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NHERF1
  • Species
    H. sapiens (human)
  • Entrez Gene
    SLC9A3R1 (a.k.a. EBP50, NHERF, NHERF-1, NHERF1, NPHLOP2)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

This plasmid contains a mutation (N268S) in the NHERF1 sequence, which corresponds to the mouse NHERF1 sequence at this position. The depositing scientist states that the mutation does not affect NHERF1 function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-NHERF1-FL was a gift from Maria-Magdalena Georgescu (Addgene plasmid # 28291 ; http://n2t.net/addgene:28291 ; RRID:Addgene_28291)
  • For your References section:

    NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands. Morales FC, Takahashi Y, Momin S, Adams H, Chen X, Georgescu MM. Mol Cell Biol. 2007 Apr . 27(7):2527-37. 10.1128/MCB.01372-06 PubMed 17242191