Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28296||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationContains aa 148-298 (deletion of the first PDZ domain and the EB domain)
Entrez GeneSLC9A3R1 (a.k.a. EBP50, NHERF, NHERF-1, NHERF1, NPHLOP2)
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-NHERF1-PDZ2 was a gift from Maria-Magdalena Georgescu (Addgene plasmid # 28296 ; http://n2t.net/addgene:28296 ; RRID:Addgene_28296)
For your References section:NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands. Morales FC, Takahashi Y, Momin S, Adams H, Chen X, Georgescu MM. Mol Cell Biol. 2007 Apr . 27(7):2527-37. 10.1128/MCB.01372-06 PubMed 17242191