pMAX deltaN-ERG
(Plasmid #29447)

• Depositing Lab: Valeri Vasioukhin
• Publication: Klezovitch et al Proc Natl Acad Sci U S A. 2008 Feb 12. 105(6):2105-10.

Backbone

• Vector backbone: pMAX-DEST
• Backbone manufacturer: Amaxa
• Backbone size w/o insert (bp): 2800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ERG
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: Deletion of first 33 aa of ERG
• Entrez Gene: ERG (a.k.a. LMPHM14, erg-3, p55)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-Fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PCR fragment amplified using IMAGE clone 6052140. Cloned into pCR8/GW/TOPO and then moved to pMAX-DEST using Gateway.

For transient expression of human deltaN-ERG. Has endogenous stop codon (no V5).