pMX155
(Plasmid #29481)

• Depositing Lab: Linda Wordeman
• Publication: Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Backbone

• Vector backbone: PEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4706
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kif2A-alpha
• Species: M. musculus (mouse)
• Insert Size (bp): 2152
• Mutation: E140K
• GenBank ID: D12644
• Entrez Gene: Kif2a (a.k.a. C530030B14Rik, Kif2, Kns2, M-kinesin)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV_fwd_primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor believes the mutation in Kif2a is the results of either errors in database sequences or polymorphisms.