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pMX156
(Plasmid #29482)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29482 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4706
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kif2A-beta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1981
  • GenBank ID
    Y15894
  • Entrez Gene
    Kif2a (a.k.a. C530030B14Rik, Kif, Kif2, Kn, Kns2, M-ki, M-kinesin)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV_fwd_primer
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

There is 1 gap and 1 insertion with depositor's reference sequence which results in missing aa R73,A74 and inserted aa AR after aa91 of Kif2a. Depositor believes the mutations in Kif2a are the results of either errors in database sequences or polymorphisms.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX156 was a gift from Linda Wordeman (Addgene plasmid # 29482 ; http://n2t.net/addgene:29482 ; RRID:Addgene_29482)
  • For your References section:

    MCAK associates with the tips of polymerizing microtubules. Moore AT, Rankin KE, von Dassow G, Peris L, Wagenbach M, Ovechkina Y, Andrieux A, Job D, Wordeman L. J Cell Biol. 2005 May 9. 169(3):391-7. 10.1083/jcb.200411089 PubMed 15883193