|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||29518||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4401
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1245
MutationN-terminal 415 aa
/ Fusion Protein
- Thrombin Cleavage Site (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Note that the 1st 12aa of Cul1 are missing. This region is disordered in the crystal structure.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAL-hCul1-NTD was a gift from Ning Zheng (Addgene plasmid # 29518 ; http://n2t.net/addgene:29518 ; RRID:Addgene_29518)