Skip to main content
Addgene
Open Menu

pSG5-CRKL
(Plasmid #29560)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29560 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 29560-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 29560-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4076
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRKL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • GenBank ID
    NM_005207
  • Entrez Gene
    CRKL

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SMAI (destroyed during cloning)
  • 3′ cloning site SMAI (destroyed during cloning)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Crkl pSG5. This construct encodes the human adapter protein Crkl. Crkl is a protein that contains one SH2 domain and two SH3 domains. It can become tyrosine phosphorylated by the c-Abl tyrosine kinase on residue Y207. Crkl is located on chromosome 22q11.21. Gene ID: 1399. HPRD: 03596. NM_005207.
The construct was made by Joanne ten Hoeve in 1992/1993.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-CRKL was a gift from Nora Heisterkamp (Addgene plasmid # 29560 ; http://n2t.net/addgene:29560 ; RRID:Addgene_29560)

  • For your References section:

    CRK proto-oncogene maps to human chromosome band 17p13. Fioretos T, Heisterkamp N, Groffen J, Benjes S, Morris C. Oncogene. 1993 Oct . 8(10):2853-5. PubMed 8378094
Related items:
Commonly requested with: