pSG5-Crkl mut SH2
(Plasmid
#41703)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41703 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSG5
- Backbone size w/o insert (bp) 4100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCrkl
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SpeciesH. sapiens (human)
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Insert Size (bp)1600
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MutationR39K
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GenBank IDNM_005207
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Entrez GeneCRKL
- Promoter SV40
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer Sv40 poly A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructed by Ron de Jong. This construct lacks some of the 5' UT sequences. A Bgl II site on either end of the insert allows the CRKL cDNA to be rmeoved as one piece.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5-Crkl mut SH2 was a gift from Nora Heisterkamp (Addgene plasmid # 41703 ; http://n2t.net/addgene:41703 ; RRID:Addgene_41703)
Map uploaded by the depositor.
