|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||29560||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4076
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1600
- Cloning method Restriction Enzyme
- 5′ cloning site SMAI (destroyed during cloning)
- 3′ cloning site SMAI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Crkl pSG5. This construct encodes the human adapter protein Crkl. Crkl is a protein that contains one SH2 domain and two SH3 domains. It can become tyrosine phosphorylated by the c-Abl tyrosine kinase on residue Y207. Crkl is located on chromosome 22q11.21. Gene ID: 1399. HPRD: 03596. NM_005207.
The construct was made by Joanne ten Hoeve in 1992/1993.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSG5-CRKL was a gift from Nora Heisterkamp (Addgene plasmid # 29560 ; http://n2t.net/addgene:29560 ; RRID:Addgene_29560)
For your References section:CRK proto-oncogene maps to human chromosome band 17p13. Fioretos T, Heisterkamp N, Groffen J, Benjes S, Morris C. Oncogene. 1993 Oct . 8(10):2853-5. PubMed 8378094