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Depositing Lab
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Depositing OrganizationUniversity of Wisconsin, Madison
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29573 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 29573-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 29573-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 4704
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWASP-interacting protein
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Alt nameWIP
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SpeciesH. sapiens (human)
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Insert Size (bp)1512
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Entrez GeneWIPF1 (a.k.a. PRPL-2, WAS2, WASPIP, WIP)
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFlag-hWIP was provided by Scott Weed (University of West Virginia)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning of the pmCherry-C1 vector has been previously described Lokuta et al., 2007.
pmCherry-C1 was generated by PCR amplification of pRSET-B-mCherry (a generous gift from Dr. Roger Tsien) with primers containing a 5' NheI site and 3' BglII site and subcloning into pEGFP-C1 (Clontech) to replace the enhanced GFP (EGFP) sequence
DNA (Catalog # 29573-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 29573-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCherryC1 Human WIP was a gift from Anna Huttenlocher (Addgene plasmid # 29573 ; http://n2t.net/addgene:29573 ; RRID:Addgene_29573) -
For your References section:
Actin-binding protein-1 interacts with WASp-interacting protein to regulate growth factor-induced dorsal ruffle formation. Cortesio CL, Perrin BJ, Bennin DA, Huttenlocher A. Mol Biol Cell. 2010 Jan 1. 21(1):186-97. 10.1091/mbc.e09-02-0106 PubMed 19910490
