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pmCherryC1 Human WIP
(Plasmid #29573)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29573 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4704
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    WASP-interacting protein
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    WIPF1 (a.k.a. PRPL-2, WAS2, WASPIP, WIP)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Flag-hWIP was provided by Scott Weed (University of West Virginia)
  • Articles Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning of the pmCherry-C1 vector has been previously described Lokuta et al., 2007.
pmCherry-C1 was generated by PCR amplification of pRSET-B-mCherry (a generous gift from Dr. Roger Tsien) with primers containing a 5' NheI site and 3' BglII site and subcloning into pEGFP-C1 (Clontech) to replace the enhanced GFP (EGFP) sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherryC1 Human WIP was a gift from Anna Huttenlocher (Addgene plasmid # 29573 ; ; RRID:Addgene_29573)
  • For your References section:

    Actin-binding protein-1 interacts with WASp-interacting protein to regulate growth factor-induced dorsal ruffle formation. Cortesio CL, Perrin BJ, Bennin DA, Huttenlocher A. Mol Biol Cell. 2010 Jan 1. 21(1):186-97. 10.1091/mbc.e09-02-0106 PubMed 19910490