pGEX-KG-WASP-GBD
(Plasmid #30113)

• Depositing Lab: David Sacks
• Publication: Kim et al J Biol Chem. 2000 Nov 24. 275(47):36999-7005.

Backbone

• Vector backbone: pGEX-KG
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 5006
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: WASP-GBD
• Alt name: WASP
• Species: H. sapiens (human)
• Insert Size (bp): 240
• Mutation: Contains the Cdc42-interacting domain of WASP (aaresidues 228-298), called WASP-GBD (for GTPase-binding domain)
• Entrez Gene: WAS (a.k.a. IMD2, SCNX, THC, THC1, WASP, WASPA)
• Entrez Gene: WAS (a.k.a. IMD2, SCNX, THC, THC1, WASP, WASPA)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pGEX5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry