|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30393||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsPlasmid is provided in DH5a cells. BL21-CodonPlus (DE3)-RILP Competent Cells from Agilent are recommended for expression.
Copy numberHigh Copy
Gene/Insert nameHJURP CenpA Binding Domain
SpeciesH. sapiens (human), S. cerevisiae (budding yeast)
Insert Size (bp)300
Entrez GeneCENPA (a.k.a. CENP-A, CenH3)
/ Fusion Protein
- His (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 pro
- 3′ sequencing primer T7 ter (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-HJURP-CBP was a gift from Yawen Bai (Addgene plasmid # 30393 ; http://n2t.net/addgene:30393 ; RRID:Addgene_30393)
For your References section:Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3. Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins LM, Xiao H, Tjandra N, Wu C, Bai Y. Nature. 2011 Apr 14. 472(7342):234-7. 10.1038/nature09854 PubMed 21412236