Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET-HJURP-CBP
(Plasmid #30393)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 30393 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmid is provided in DH5a cells. BL21-CodonPlus (DE3)-RILP Competent Cells from Agilent are recommended for expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HJURP CenpA Binding Domain
  • Species
    H. sapiens (human), S. cerevisiae (budding yeast)
  • Insert Size (bp)
    300
  • Entrez Gene
    CENPA (a.k.a. CENP-A, CenH3)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 pro
  • 3′ sequencing primer T7 ter
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-HJURP-CBP was a gift from Yawen Bai (Addgene plasmid # 30393 ; http://n2t.net/addgene:30393 ; RRID:Addgene_30393)
  • For your References section:

    Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3. Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins LM, Xiao H, Tjandra N, Wu C, Bai Y. Nature. 2011 Apr 14. 472(7342):234-7. 10.1038/nature09854 PubMed 21412236