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786 pCSmAKT2Acherry
(Plasmid #31167)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31167 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Expression vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Akt
  • Alt name
    viral 2A peptide
  • Mutation
    membrane tethered Akt
  • Tags / Fusion Proteins
    • Myr (N terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expression clone for generating mRNA encoding membrane tethered Akt in frame with the viral 2A peptide and mCherry.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    786 pCSmAKT2Acherry was a gift from Nathan Lawson (Addgene plasmid # 31167 ; http://n2t.net/addgene:31167 ; RRID:Addgene_31167)
  • For your References section:

    A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development. Covassin LD, Siekmann AF, Kacergis MC, Laver E, Moore JC, Villefranc JA, Weinstein BM, Lawson ND. Dev Biol. 2009 May 15. 329(2):212-26. 10.1016/j.ydbio.2009.02.031 PubMed 19269286