Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31203||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepLenti 6/V5-DEST
- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsGrow in STBL3 bacteria (Invitrogen) at lower temps (~30C) to prevent recombination and increase plasmid production.
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1218
Entrez GeneANLN (a.k.a. FSGS8, Scraps, scra)
/ Fusion Protein
- V5 (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (destroyed during cloning)
- 3′ cloning site attR2 (destroyed during cloning)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer V5 Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that the ANLN insert in this plasmid is based on GenBank entry BC034692, rather than the full-length transcript (GenBank entry NM_018685.2).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti6/V5-DEST-ANLN was a gift from Lynda Chin (Addgene plasmid # 31203 ; http://n2t.net/addgene:31203 ; RRID:Addgene_31203)
For your References section:Proinvasion metastasis drivers in early-stage melanoma are oncogenes. Scott KL, Nogueira C, Heffernan TP, van Doorn R, Dhakal S, Hanna JA, Min C, Jaskelioff M, Xiao Y, Wu CJ, Cameron LA, Perry SR, Zeid R, Feinberg T, Kim M, Vande Woude G, Granter SR, Bosenberg M, Chu GC, DePinho RA, Rimm DL, Chin L. Cancer Cell. 2011 Jul 12;20(1):92-103. 10.1016/j.ccr.2011.05.025 PubMed 21741599