PurposeMitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafish
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31241||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namemitochondrial localization enhanced green fluorescent protein
SpeciesD. rerio (zebrafish)
- Cloning method Restriction Enzyme
- 5′ cloning site HIndIII (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSCAC-69 was a gift from Seok-Yong Choi (Addgene plasmid # 31241 ; http://n2t.net/addgene:31241 ; RRID:Addgene_31241)
For your References section:Real-time imaging of mitochondria in transgenic zebrafish expressing mitochondrially targeted GFP. Kim MJ, Kang KH, Kim CH, Choi SY. Biotechniques. 2008 Sep . 45(3):331-4. 10.2144/000112909 PubMed 18778258