|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31379||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
Gene/Insert nameYellow Cameleon 3.60
Insert Size (bp)1962
/ Fusion Proteins
- eCFP (N terminal on insert)
- Venus (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byReceived from lab of A. Miyawaki
Terms and Licenses
Article Citing this Plasmid
Optical recording of neuronal activity with a genetically-encoded calcium indicator in anesthetized and freely moving mice.
Lütcke H, Murayama M, Hahn T, Margolis DJ, Astori S, Zum Alten Borgloh SM, Göbel W, Yang Y, Tang W, Kügler S, Sprengel R, Nagai T, Miyawaki A, Larkum ME, Helmchen F, Hasan MT
Front Neural Circuits. 2010 Apr 29;4:9.
There are a few mismatches between Addgene's sequencing results and the full sequence but they do not alter plasmid function. There are two 2 nucleotide deletions corresponding to nucleotides 487-488 and 494-495. There is also a mismatch at position 791. These do not alter protein function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV-6P-SEW-YC3.6 was a gift from Fritjof Helmchen (Addgene plasmid # 31379 ; http://n2t.net/addgene:31379 ; RRID:Addgene_31379)
For your References section:Chronic imaging of cortical sensory map dynamics using a genetically encoded calcium indicator. Minderer M, Liu W, Sumanovski LT, Kugler S, Helmchen F, Margolis DJ. J Physiol. 2011 Nov 14. 10.1113/jphysiol.2011.219014 PubMed 22083602