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(Plasmid #31379)


Item Catalog # Description Quantity Price (USD)
Plasmid 31379 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy


  • Gene/Insert name
    Yellow Cameleon 3.60
  • Alt name
  • Species
    synthetic construct
  • Insert Size (bp)
  • GenBank ID
  • Tags / Fusion Proteins
    • eCFP (N terminal on insert)
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Received from lab of A. Miyawaki
  • Article Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Optical recording of neuronal activity with a genetically-encoded calcium indicator in anesthetized and freely moving mice.
Lütcke H, Murayama M, Hahn T, Margolis DJ, Astori S, Zum Alten Borgloh SM, Göbel W, Yang Y, Tang W, Kügler S, Sprengel R, Nagai T, Miyawaki A, Larkum ME, Helmchen F, Hasan MT
Front Neural Circuits. 2010 Apr 29;4:9.

There are a few mismatches between Addgene's sequencing results and the full sequence but they do not alter plasmid function. There are two 2 nucleotide deletions corresponding to nucleotides 487-488 and 494-495. There is also a mismatch at position 791. These do not alter protein function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-6P-SEW-YC3.6 was a gift from Fritjof Helmchen (Addgene plasmid # 31379 ; ; RRID:Addgene_31379)
  • For your References section:

    Chronic imaging of cortical sensory map dynamics using a genetically encoded calcium indicator. Minderer M, Liu W, Sumanovski LT, Kugler S, Helmchen F, Margolis DJ. J Physiol. 2011 Nov 14. 10.1113/jphysiol.2011.219014 PubMed 22083602