Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31527||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameYFP-HA-Protein Kinase D
SpeciesH. sapiens (human)
Entrez GenePRKD1 (a.k.a. CHDED, PKC-MU, PKCM, PKD, PRKCM)
/ Fusion Proteins
- YFP (N terminal on insert)
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It should work fine with the 12CA5 HA antibody.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-YFP-PKD1 was a gift from Alexandra Newton (Addgene plasmid # 31527 ; http://n2t.net/addgene:31527 ; RRID:Addgene_31527)
For your References section:The protein scaffold NHERF-1 controls the amplitude and duration of localized protein kinase D activity. Kunkel MT, Garcia EL, Kajimoto T, Hall RA, Newton AC. J Biol Chem. 2009 Sep 4. 284(36):24653-61. 10.1074/jbc.M109.024547 PubMed 19581308
Map uploaded by the depositor.