Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31619||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerHawley et al., 1994
- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression, Retroviral
Selectable markerspuromycin N-acetyl transferase (pac)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneSmo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
/ Fusion Protein
- SNAP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV-SNAP-Smo was a gift from Rajat Rohatgi (Addgene plasmid # 31619 ; http://n2t.net/addgene:31619 ; RRID:Addgene_31619)
For your References section:Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium. Milenkovic L, Scott MP, Rohatgi R. J Cell Biol. 2009 Nov 2. 187(3):365-74. 10.1083/jcb.200907126 PubMed 19948480