pDONR221-MICU1-mEF
(Plasmid
#31765)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31765 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 1874
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Vector typepDONR Gateway
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMICU1
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Alt nameCBARA1
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SpeciesH. sapiens (human)
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Insert Size (bp)1431
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MutationMutated in both EF-hand domains: D231A, E242K, D421A, E432K. Also, there are 8 synonymous changes in b.p. 1272-1293 downstream from ATG for RNAi resistance.
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GenBank IDNM_001195518
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Entrez GeneMICU1 (a.k.a. CALC, CBARA1, EFHA3, MPXPS, ara CALC)
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Tag
/ Fusion Protein
- None
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 (-21) Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-MICU1-mEF was a gift from Vamsi Mootha (Addgene plasmid # 31765 ; http://n2t.net/addgene:31765 ; RRID:Addgene_31765) -
For your References section:
MICU1 encodes a mitochondrial EF hand protein required for Ca(2+) uptake. Perocchi F, Gohil VM, Girgis HS, Bao XR, McCombs JE, Palmer AE, Mootha VK. Nature. 2010 Sep 16;467(7313):291-6. Epub 2010 Aug 8. 10.1038/nature09358 PubMed 20693986
