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pRS316-GFP-COF1
(Plasmid #31843)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31843 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS316
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 4887
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cofilin
  • Alt name
    Cof1p
  • Alt name
    COF1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    COF1 (a.k.a. YLL050C)
  • Promoter T7
  • Tag / Fusion Protein
    • GFP (S65T)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GFP-cofilin construct has S65T-GFP and a 12–amino acid linker (GHGTGSTGSGSS) inserted in between amino acids N74 and G75 of cofilin. Note that the GFP is NOT N-terminal.

A missense mutation was detected by the lab in sequencing: converting the third glycine in the 5′ GFP linker into an arginine. This was the construct used in the paper.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS316-GFP-COF1 was a gift from David Drubin (Addgene plasmid # 31843 ; http://n2t.net/addgene:31843 ; RRID:Addgene_31843)
  • For your References section:

    Cofilin recruitment and function during actin-mediated endocytosis dictated by actin nucleotide state. Okreglak V, Drubin DG. J Cell Biol. 2007 Sep 24;178(7):1251-64. Epub 2007 Sep 17. 10.1083/jcb.200703092 PubMed 17875745