|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31947||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5553
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt namephotoactivatable dark-to-red TagRFP red fluorescent protein
Insert Size (bp)714
MutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H203R/R207I/V218W/C229S compared to TagRFP (but numbering relative to EGFP)
- Promoter CMV
/ Fusion Protein
- VSVG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer cggtaggcgtgtacggtgggag (Common Sequencing Primers)
Please note that this construct was used in the original publication for localization studies and not for producing virus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pVSVG-PATagRFP was a gift from Vladislav Verkhusha (Addgene plasmid # 31947 ; http://n2t.net/addgene:31947 ; RRID:Addgene_31947)
For your References section:Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells. Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV. J Am Chem Soc. 2010 May 12;132(18):6481-91. 10.1021/ja100906g PubMed 20394363