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MCV-Rep-
(Plasmid #32058)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32058 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 32058-D50 50 μg of DNA in Tris buffer $495
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DNA 32058-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pJ241
  • Backbone manufacturer
    DNA2.0
  • Backbone size w/o insert (bp) 2755

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCV-Rep- genome
  • Species
    Merkel cell polyomavirus
  • Insert Size (bp)
    5387
  • Mutation
    Single cytosine to adenine (C/A) mutation at 2,073 (nt) of full construct, which deletes viral replication function
  • GenBank ID
    JF813003
  • Promoter none from vector. Insert contains native promoter from viral genome encompassed in the viral non-coding control region.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrFI (not destroyed)
  • 3′ cloning site BsrFI (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MCV-Rep- was a gift from Patrick Moore (Addgene plasmid # 32058 ; http://n2t.net/addgene:32058 ; RRID:Addgene_32058)

  • For your References section:

    Cellular and viral factors regulating merkel cell polyomavirus replication. Feng H, Kwun HJ, Liu X, Gjoerup O, Stolz DB, Chang Y, Moore PS. PLoS One. 2011;6(7):e22468. Epub 2011 Jul 22. 10.1371/journal.pone.0022468 PubMed 21799863
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