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pADL*
(Plasmid #32097)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32097 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMONO-zeo
  • Backbone manufacturer
    InvivoGen
  • Backbone size w/o insert (bp) 3429
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin(Zeocin)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCV Large T antigen
  • Alt name
    MCPyV
  • Alt name
    Merkel Cell Polyomavirus
  • Species
    virus
  • Insert Size (bp)
    2453
  • Mutation
    57kT splice sites silently mutated, P156S to match consensus

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer TGAGCGGAGCTAATTCTCG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please consult laboratory website, http://home.ccr.cancer.gov/Lco/plasmids.asp, for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pADL* was a gift from Christopher Buck (Addgene plasmid # 32097)
  • For your References section:

    Glycosaminoglycans and sialylated glycans sequentially facilitate merkel cell polyomavirus infectious entry. Schowalter RM, Pastrana DV, Buck CB. PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28. 10.1371/journal.ppat.1002161 PubMed 21829355